2-1 Hirosawa, Wako, Saitama 351-0198, Japan

SELECTED PUBLICATIONS

  • Sangamithirai Subramanian Parimalam, Yusuke Oguchi, Mahmoud Abdelmoez, Arata Tsuchida, Yuka Ozaki, Ryuji Yokokawa, Hidetoshi Kotera, and Hirofumi Shintaku, Electrical Lysis and RNA Extraction from Single Cells Fixed by Dithio-bis(succinimidyl propionate), Analytical Chemistry, Vol. 90, No. 21 (2018), pp.12512-12518.

  • Mahmoud N. Abdelmoez,* Kei Iida,* Yusuke Oguchi,* Hidekazu Nishikii, Ryuji Yokokawa, Hidetoshi Kotera, Sotaro Uemura, Juan G. Santiago, and Hirofumi Shintaku, SINC-seq: Correlation of Transient Gene Expressions between Nucleus and Cytoplasm Reflects Single-cell Physiology, Genome Biology, 19 (2018), 66, https://doi.org/10.1186/s13059-018-1446-9. [*: Equal contributors]

  •  Kentaro Kuriyama,* Hirofumi Shintaku,* and Juan G. Santiago, Isotachophoresis for Fractionation and Recovery of Cytoplasmic RNA and Nucleus from Single Cells, Electrophoresis, Vol. 36, Issue 14(2015) pp. 1658-1662. [*: Equal contributors]

  • Hirofumi Shintaku, James W. Palko, Glenn M. Sanders, and Juan G. Santiago, Increasing Hybridization Rate and Sensitivity of Bead Based Assays Using Isotachophoresis, Angewandte Chemie International Edition, Vol. 53 (2014), pp. 13813-13816.

  • Hirofumi Shintaku, Hidekazu Nishikii, Lewis A. Marshall, Hidetoshi Kotera, and Juan G. Santiago, On-Chip Separation and Analysis of RNA and DNA from Single Cells, Analytical Chemistry, Vol. 86, No. 4 (2014), pp 1953–1957.

 

JOURNAL PUBLICATIONS (as of March 3rd, 2019)

  1. Taikopaul Kaneko, Suguru Ando, Ken'ya Furuta, Kazuhiro Oiwa, Hirofumi Shintaku, Hidetoshi Kotera, and Ryuji Yokokawa, Transport of microtubules according to the number and spacing of kinesin motors on gold nano-pillars, Nanoscale (2019), 10.1039/C9NR01324E.

  2. Kazuya Fujimoto, Yuki Morita, Ryota Iino, Michio Tomoshige, Hirofumi Shintaku, Hidetoshi Kotera, and Ryuji Yokokawa, Simultaneous Observation of Kinesin-Driven Microtubule Motility and Binding of Adenosine Triphosphate Using Linear Zero-Mode Waveguides, ACS NANO, Vo.12, No.12 (2018), pp.11975-11985.

  3. Sangamithirai Subramanian Parimalam, Yusuke Oguchi, Mahmoud Abdelmoez, Arata Tsuchida, Yuka Ozaki, Ryuji Yokokawa, Hidetoshi Kotera, and Hirofumi Shintaku, Electrical Lysis and RNA Extraction from Single Cells Fixed by Dithio-bis(succinimidyl propionate), Analytical Chemistry, Vol. 90, No. 21 (2018), pp.12512-12518.

  4. Ruba Khnouf,* Sabrina Shore,* Crystal M. Han, Jordana M. Henderson, Sarah A. Munro, Anton P. McCaffrey, Hirofumi Shintaku,† and Juan G. Santiago,† Efficient Production of On-target Reads for Small RNA Sequencing of Single Cells Using Modified Adapters, Analytical Chemistry, Vol. 90, No. 21 (2018), pp.12609-12615. [*: Equal contributors, †: Corresponding authors]

  5. Nao Nitta, Takeaki Sugimura, Akihiro Isozaki, Hideharu Mikami, Kei Hiraki, Shinya Sakuma, Takanori Iino, Fumihito Arai, Taichiro Endo, Yasuhiro Fujiwaki, Hideya Fukuzawa, Misa Hase, Takeshi Hayakawa, Kotaro Hiramatsu, Yu Hoshino, Mary Inaba, Takuro Ito, Hiroshi Karakawa, Yusuke Kasai, Kenichi Koizumi, SangWook Lee, Cheng Lei, Ming Li, Takanori Maeno, Satoshi Matsusaka, Daichi Murakami, Atsuhiro Nakagawa, Yusuke Oguchi, Minoru Oikawa, Tadataka Ota, Kiyotaka Shiba, Hirofumi Shintaku, Yoshitaka Shirasaki, Kanako Suga, Yuta Suzuki, Nobutake Suzuki, Yo Tanaka, Hiroshi Tezuka, Chihana Toyokawa, Yaxiaer Yalikun, Makoto Yamada, Mai Yamagishi, Takashi Yamano, Atsushi Yasumoto, Yutaka Yatomi, Masayuki Yazawa, Dino Di Carlo, Yoichiroh Hosokawa, Sotaro Uemura, Yasuyuki Ozeki, Keisuke Goda, Intelligent Image-Activated Cell Sorting, Cell, 175 (2018), https://doi.org/10.1016/j.cell.2018.08.028.

  6. Mahmoud N. Abdelmoez,* Kei Iida,* Yusuke Oguchi,* Hidekazu Nishikii, Ryuji Yokokawa, Hidetoshi Kotera, Sotaro Uemura, Juan G. Santiago, and Hirofumi Shintaku, SINC-seq: Correlation of Transient Gene Expressions between Nucleus and Cytoplasm Reflects Single-cell Physiology, Genome Biology, 19 (2018), 66, https://doi.org/10.1186/s13059-018-1446-9 . [*: Equal contributors]

  7. Naoto Isozaki, Hirofumi Shintaku, Hidetoshi Kotera, Taviare L. Hawkins, Jennifer L. Ross, and Ryuji Yokokawa, Control of Molecular Shuttles by Designing Electrical and Mechanical Properties of Microtubules, Science Robotics, Vol. 2, Issue 10 (2017), eaan4882.

  8. Subhathirai Subramaniyan Parimalam, Mehmet C. Tarhan, Stanislav L. Karsten, Hiroyuki Fujita, Hirofumi Shintaku, Hidetoshi Kotera, and Ryuji Yokokawa, Microtubule Density and Landing Rate as Parameters to Analyze Tau Protein in the MT-kinesin “Gliding” Assay, Sensors and Actuators B: Chemical, Vol. 238, (2017), pp. 954-961.

  9. 藤本 和也, 森田 有貴, 飯野 亮太, 富重 道雄, 新宅 博文, 小寺 秀俊, 横川 隆司, Linear-shaped Zero Mode Waveguidesの設計・製作とそれを用いたキネシンおよびATPの1分子観察, 電気学会論文誌E(センサ・マイクロマシン部門誌), 137巻6号(2017), pp. 159-164.

  10. Kentaro Kuriyama,* Hirofumi Shintaku,* and Juan G. Santiago, Protocol for Microfluidic System to Automate the Preparation and Fractionation of the Nucleic Acids in the Cytoplasm versus Nuclei of Single Cells, Bio-protocol, Vol. 6, Issue 12, (2016), e1844. [*: Equal contributors]

  11. Kazuya Fujimoto, Hirofumi Shintaku, Hidetoshi Kotera, and Ryuji Yokokawa, Pneumatically-driven Microfluidic Device for Evaluating Active Transport by Kinesin Motor Protein, IEEJ Transactions on Sensors and Micromachines, Vol. 136, Issue 9 (2016), pp.384-389.

  12. Subhathirai Subramaniyan Parimalam, Mehmet C. Tarhan, Stanislav L. Karsten, Hiroyuki Fujita, Hirofumi Shintaku, Hidetoshi Kotera, and Ryuji Yokokawa, On-chip Microtubule Gliding Assay for Parallel Measurement of Tau Protein Species, Lab on a Chip, Vol. 16, Issue 9 (2016), pp.1691-1697.

  13. Tasuku Nakahara, Hirofumi Shintaku, Hidetoshi Kotera, and Ryuji Yokokawa, Velocity Control of Microtubules with High Spatial Resolution on an Au-coated Surface with an SU-8 Thermal Isolation Layer, IEEJ Transactions on Sensors and Micromachines, Vol. 136, Issue 3 (2016), pp. 77-82.

  14. Kentaro Kuriyama,* Hirofumi Shintaku,* and Juan G. Santiago, Isotachophoresis for Fractionation and Recovery of Cytoplasmic RNA and Nucleus from Single Cells, Electrophoresis, Vol. 36, Issue 14(2015) pp. 1658-1662. [*: Equal contributors]

  15. Kazuya Fujimoto, Moeto Nagai, Hirofumi Shintaku, Hidetoshi Kotera, and Ryuji Yokokawa, Dynamic Formation of a Microchannel Array Enabling Kinesin-Driven Microtubule Transport between Separate Compartments on a Chip, Lab on a Chip, Vol.15, No.9 (2015), pp. 2055-2063.

  16. Itsuo Hanasaki, Naoya Yukimoto, Satoshi Uehara, Hirofumi Shintaku, and Satoyuki Kawano, Linearization of Lambda DNA Molecules by Instantaneous Variation of the Trapping Electrode Voltage inside a Micro-channel, Journal of Physics D: Applied Physics Vol.48, No.13 (2015) 135402 (11 pages).

  17. Naoto Isozaki, Suguru Ando, Tasuku Nakahara, Hirofumi Shintaku, Hidetoshi Kotera, Edgar Meyhöfer, and Ryuji Yokokawa, Control of Microtubule Trajectory within an Electric Field by Altering Surface Charge Density, Scientific Reports, 5 (2015), 7669 (8 pages).

  18. Hirofumi Shintaku, James W. Palko, Glenn M. Sanders, and Juan G. Santiago, Increasing Hybridization Rate and Sensitivity of Bead Based Assays Using Isotachophoresis, Angewandte Chemie International Edition,Vol. 53 (2014), pp. 13813-13816.

  19. Hirofumi Shintaku, Hidekazu Nishikii, Lewis A. Marshall, Hidetoshi Kotera, and Juan G. Santiago, On-Chip Separation and Analysis of RNA and DNA from Single Cells, Analytical Chemistry, Vol. 86, No. 4 (2014), pp 1953–1957.

  20. Hirofumi Shintaku, Kazumi Hakamada, Hiroshi Fujimoto, Takeshi Nagata, Jun Miyake, and Satoyuki Kawano, Measurement of Local Electric Field in Microdevices for Low-Voltage Electroporation of Adherent Cells, Microsystem Technologies, Vol. 20, Issue 2 (2014), pp. 303-313.

  21. Tasuku Nakahara, Junya Ikuta, Hirofumi Shintaku, Hidetoshi Kotera, Ryuji Yokokawa, In Situ Velocity Control of Gliding Microtubules with Temperature Monitoring by Fluorescence Excitation on a Patterned Gold Thin Film, Materials Research Express, Vol.1, No.4 (2014), 045405 (12 pages).

  22. Junya Ikuta, Nagendra K. Kamisetty, Hirofumi Shintaku, Hidetoshi Kotera, Takahide Kon, and Ryuji Yokokawa, Tug-of-War of Microtubule Filaments at the Boundary of a Kinesin- and Dynein-Patterned Surface, Scientific Reports, Vol.4 (2014), 5281 (8 pages).

  23. Tasuku Nakahara, Naoto Isozaki, Suguru Ando, Nagendra Kumar Kamisetty, Hirofumi Shintaku, Hidetoshi Kotera, Ryuji Yokokawa, Fabrication of a Perfusable Glass Microfluidic Channel for Microtubule Manipulation Using an Electric Field, IEEJ Transactions on Sensors and Micromachines, Vol. 134, No. 3 (2014), pp. 64-69.

  24. Hirofumi Shintaku, Takayuki Kobayashi, Kazuki Zusho, Hidetoshi Kotera, and Satoyuki Kawano, Wide-Range Frequency Selectivity in Acoustic Sensor Fabricated Using Microbeam Array with Non-uniform Thickness, Journal of Micromechanics and Microengineering, Vol. 23, No.11 (2013), 115014 (7 pages).

  25. Hirofumi Shintaku, Takatoshi Inaoka, Takayuki Nakagawa, Satoyuki Kawano, and Juichi Ito, Electrically Evoked Auditory Brainstem Response by Using Bionic Auditory Membrane in Guinea Pigs, Journal of Biomechanical Science and Engineering, Vol. 8, No. 3 (2013), pp. 198-208.

  26. Takashi Tateno, Jun Nishikawa, Nobuyoshi Tsuchioka, Hirofumi Shintaku, and Satoyuki Kawano, A Hardware Model of the Auditory Periphery to Transduce Acoustic Signals into Neural Activity, Frontiers in Neuroengineering, Vol. 6 (2013), doi:103389/fneng.1013.00012.

  27. Kazumi Hakamada, Hirofumi Shintaku, Takeshi Nagata, Hiroshi Fujimoto, Satoyuki Kawano, and Jun Miyake, Development of Microfabricated Device for Low-Voltage Electroporation of Adherent Cells, Journal of Bioscience and Bioengineering, Vol. 115, Issue 3 (2013), pp. 314-319.

  28. Naoya Yukimoto, Makusu Tsutsui, Yuhui He, Hirofumi Shintaku, Syoji Tanaka, Satoyuki Kawano, Tomoji Kawai, and Masateru Taniguchi, Tracking Single-Particle Dynamics via Combined Optical and Electrical Sensing, Scientific Reports, Vol. 3 (2013), 1855 (7 pages).

  29. Osman Omran Osman, Hrofumi Shintaku, and Satoyuki Kawano, Development of Micro-Vibrating Flow Pumps Using MEMS Technologies, Microfluidics and Nanofluidics, Vol. 13, Issue 5 (2012), pp. 703-713.

  30. Satoshi Uehara, Hirofumi Shintaku, and Satoyuki Kawano, Electrokinetic Flow Dynamics of Weakly Aggregated lDNA Confined in Nanochannels, Journal of Fluids Engineering, Transaction of the ASME, Vol. 133, Issue 12 (2011), 121203 (8 pages).

  31. Takatoshi Inaoka*, Hirofumi Shintaku*, Takayuki Nakagawa, Satoyuki Kawano, Hideaki Ogita, Tatsunori Sakamoto, Shinji Hamanishi, Hiroshi Wada, and Juichi Ito, Piezoelectric Materials Mimic the Function of the Cochlear Sensory Epithelium, Proceedings of the National Academy of Sciences of the United States of America, Vol. 108, No. 45 (2011), pp. 18390-18395. [*: Equal contributors]

  32. Hirofumi Shintaku, Tsubasa Yonemura, Kazuaki Tsuru, Takashi Isoyama, Tomoyuki Yambe, and Satoyuki Kawano, Oxygenation to Bovine Blood in Artificial Heart and Lung Using Vibrating Flow Pump: Experiment and Numerical Analysis Based on Non-Newtonian Model, Journal of Fluid Science and Technology, Vol. 5, No. 2 (2010), pp. 292-304.

  33. Hirofumi Shintaku, Takashi Tateno, Nobuyoshi Tsuchioka, Harto Tanujaya, Takayuki Nakagawa, Juichi Ito, and Satoyuki Kawano, Culturing Neurons on MEMS Fabricated P(VDF-TrFE) Films for Implantable Artificial Cochlea, Journal of Biomechanical Science and Engineering, Vol. 5, No. 3 (2010), pp. 229-235.

  34. Hirofumi Shintaku, Takayuki Nakagawa, Dai Kitagawa, Harto Tanujaya, Satoyuki Kawano, and Juichi Ito, Development of Piezoelectric Acoustic Sensor with Frequency Selectivity for Artificial Cochlea, Sensors and Actuators A: Physical, Vol. 158, Issue 2 (2010), pp. 183-192.

  35. Kentaro Doi, Tomoaki Haga, Hirofumi Shintaku, and Satoyuki Kawano, Developments of Coarse Graining DNA Models for Single Nucleotide Resolution Analysis, Philosophical Transactions of the Royal Society A, Vol. 368, Issue 1920 (2010), pp. 2615-2628.

  36. Hirofumi Shintaku, Shuji Azuma, and Satoyuki Kawano, Measurements of Electric Field and Electrokinetic Phenomena Using Two Kinds of Tracer Particles with Different Mobilities, Journal of Fluid Science and Technology, Vol. 4, No. 3 (2009), pp. 687-698.

  37. 新宅 博文,東 修士,川野 聡恭,移動度の異なるトレーサ粒子を用いた電場と界面動電現象の計測,日本機械学会論文集(B編), 第75巻, 753号 (2009), pp. 945-952.

  38. Hirofumi Shintaku, Yukio Tatara, and Satoyuki Kawano, Droplet Transportation on Vertical Parallel Electrodes Using Electrowetting and Interfacial Oscillation, Journal of Fluid Science and Technology, Vol. 4, No. 3 (2009), pp. 636-647.

  39. 新宅 博文, 多田羅 晋生, 川野 聡恭, Electrowettingおよび界面振動を用いた鉛直平行電極上における微小液滴の輸送, 日本機械学会論文集(B編), 第74巻, 745号 (2008), pp. 1942-1950.

  40. Itsuo Hanasaki, Hirofumi Shintaku, Satoshi Matsunami, and Satoyuki Kawano, Structural and Tensile Properties of Self-Assembled DNA Network on Mica Surface, Computer Modeling in Engineering & Sciences, Vol. 46, No. 2 (2009), pp. 191-207.

  41. Hirofumi Shintaku, Teru Okitsu, Satoyuki Kawano, Shinichi Matsumoto, Takaaki Suzuki, Isaku Kanno, and Hidetoshi Kotera, Effects of Fluid Dynamic Stress on Fracturing of Cell-aggregated Tissue during Purification for Islets of Langerhans Transplantation, Journal of Physics D: Applied Physics, Vol. 41, Issue 11(2008), 115507 (9 pages).

  42. Hirofumi Shintaku, Shosaku Imamura, and Satoyuki Kawano, Microbubble Formations in MEMS-Fabricated Rectangular Channels: A High-Speed Observation, Experimental Thermal and Fluid Science, Vol. 32, Issue 5 (2008), pp. 1132-1140.

  43. 新宅 博文, 米村 翼, 磯山 隆, 山家 智之, 川野 聡恭, 酸素付加能を有する振動流型人工心臓の開発とその牛血実験, 日本機械学会論文集(B編), 第74巻, 第740号 (2008), pp. 862-870.

  44. Hirofumi Shintaku, Takeo Kuwabara, Satoyuki Kawano, Takaaki Suzuki, Isaku Kanno, and Hidetoshi Kotera, Micro Cell Encapsulation and Its Hydrogel-beads Production Using Microfluidic Device, Microsystem Technologies, Vol. 13, No. 8-10 (2007), pp. 951-958.

  45. 新宅 博文, 川野 聡恭, 鈴木 孝明, 神野 伊策, 小寺 秀俊, マイクロチャネルにおける液体微粒化法とその特性解析, 日本機械学会論文集(B編), 第71巻, 708号 (2005), pp. 2007-2012.

  46. 新宅 博文, 川野 聡恭, 神野 伊策, 小寺 秀俊, μTAS用パッシブミキサーにおける流体混合と物質移動特性, 日本機械学会論文集(B編), 第71巻, 第701号 (2005), pp. 111-116.

  47. 鈴木 孝明, 秦 秀敏, 新宅 博文, 神野 伊策, 小寺 秀俊, 圧電アクチュエータを用いた進行波型バルブレスマイクロポンプの開発, 日本AEM学会誌, Vol. 13, No. 4 (2005), pp. 310-315.

BOOK CHAPTERS & OTHERS

  1.  新宅博文,1細胞解析とマイクロフルイディックスの今後, 化学とマイクロ・ナノシステム学会 会誌,Vol. 18, No.1 (2019), pp.9-10.

  2. 小口祐伴,マハムード ナディ アブデルモエズ,新宅博文,SINC-seq: 1細胞の核RNAと細胞質RNAの定量相関解析,生物物理,Vol.59, No.2,(2019), pp.88-90.

  3. 新宅 博文, 中川 隆之, 川野 聡恭, 田畑 泰彦, 和田 仁, 伊藤 壽一, “MEMS技術による人工内耳の開発”, ヘルスケアとバイオ医療のための先端デバイス機器(三林浩二監修), (2009), pp. 326-335, シーエムシー出版.(分担執筆)

  4. Takaaki Suzuki, Isaku Kanno, Hidetoshi Hata, Hirofumi Shintaku, Satoyuki Kawano, and Hidetoshi Kotera, Improving the Performance of a Traveling Wave Micropump for Fluid Transport in Micro Total Analysis Systems, Complex Medical Engineering (edited by J.L. Wu, K. Ito, S. Tobimatsu, T. Nishida, H. Fukuyama (Eds.)), Springer (2007), pp. 3-12.

PATENTS

  1. 新宅博文,藁谷卓哉,上村想太郎,小口祐伴,核酸分離用チップ、それを用いた核酸分離方法、および核酸分析方法,特願2016-014708(2016/1/28) 生体高分子分画用チップ、それを用いた生体高分子の分画方法、および生体高分子の分析方法, 特願2016-177163(2016/9/9),PCT/JP2017/003069 (2017/1/27)

  2. Juan G. Santiago, Hirofumi Shintaku, Simultaneous Extraction and Separation of RNA and DNA from Single Cells Using Electrophoretic Techniques, US 14/593,925 (2015/1/9), PCT/US2015/010884(2016/4/7)

  3. 川野 聡恭, 伊藤壽一,中川隆之,新宅博文,人工感覚上皮,特開2014-45937.