00:00:00 - 00:05:00: Angela Wu - Welcome Remarks 00:05:11 - 00:36:40: Aviv Regev & Sarah Teichmann - State of the HCA 00:37:22 - 00:58:21: Jay W. Shin & Shyam Prabhakar - HCA Asia Updates 01:00:45 - 01:45:10: Alexandra-Chloe Villani - Keynote 01:47:58 - 02:11:12: Yanyi Huang - Multiplexed Profiling of RNA In-Situ through Single-round of Imaging in Three-Dimensional Tissue 02:11:30 - 02:33:58: Hirofumi Shintaku - Nano-electrokinetic Transcriptomics to Link Gene Expressions to Single-cell Behaviors 02:33:09 - 02:47:46: Gang Cao - Integrating Single-cell Spatial-omics with Connectome

Talk 1: Opening talk selected from outstanding papers published in Microsystems & Nanoengineering: "The Microfluidic Edge: Elevating the Standards of Sperm Selection", Steven Vasilescu, University of Technology Sydney Talk 2: "Micro- and nanoscale electrokinetics for single-cell multimodal analyses", Hirofumi Shintaku, Kyoto University Talk 1 abstract: Our study introduces new microfluidic technology that transforms the way we select sperm for fertility treatments by mimicking the female reproductive tract. This biomimetic method, far surpassing traditional techniques, harnesses the power of microscale channels in a kind of sperm olympics to isolate the healthiest and most viable sperm with remarkable precision. This technology is not only innovative but also offers a gentler, quicker, and more reliable process, making it a promising advancement in reproductive medicine. Talk 2 abstract: Nanopore electroporation uses nanoscale structures to create focused electric fields, which form pores in lipid bilayers with low invasiveness. We here discuss the biophysics of the pore formation that depends on the cell surface tension and introduce an approach that leverages nanopore electroporation and single-cell RNA-sequencing to parallelly profile cell surface tension and gene expression. We show that our system dissects the heterogeneity in cellular mechanics and uncovers the regulatory mechanism in cancer malignancy, cell differentiation, and cellular senescence.

(0:00:00) Clonal lineage tracing in haematopoiesis and embryonic development (Shalin H. Naik) (0:29:22) scONE-seq: A Novel Single-cell Multiple-Omic Technology Reveals Genotype and Phenotype Heterogeneity Of A Frozen Tumor Sample (Angela Ruohao Wu) (0:45:08) Technologies for studying immunity development: single-cell analyses and beyond (Jianbin Wang) (0:59:55) Integrative Analysis of Single-cell Transcriptomics with Multiplexed RNA Imaging for Mapping Autism Pathophysiology (Jinyue Liu) (1:15:34) Exploring transcriptional noise in subcellular compartments with on-chip electrophoretic fractionation of cytoplasmic versus nuclear RNAs (Hirofumi Shintaku) (1:30:29) Heterogeneous response to, and recovery from, hypomethylating agents in AML cells (Heather Lee) (1:46:30) Spatially regularized clustering algorithm for identifying cell types and tissue domains from spatial omics data (Vipul Singhal) (2:02:18) Large field of view, high-resolution spatially resolved transcriptomics using DNA nanoball patterned array (Longqi Liu)